Clic1 plays a role in mouse hepatocarcinoma via modulating Annexin A7 and Gelsolin in vitro and in vivo

Clic1 is a member of the family of chloride intracellular ion channels. Previous studies suggest that Clic1 is involved in migration and invasion of the lymphatic metastasis in hepatocarcinoma, however, the mechanism is not fully understood. In the present study, we observed Clic1 is abundant in cytoplasm, higher expression in Hca-F cell than Hca-P cell, and we showed that downregulation of Clic1 by RNA interference was able to markedly enhance the expression of tumor metastasis genes Annexin A7 and Gelsolin in vitro, and downregulation of Annexin A7 and Gelsolin also enhanced the expression of Clic1 in vitro and in vivo. Our results provide novel insight that Clic1 have a role in migration and invasion in hepatocarcinoma maybe via modulating the expression of Annexin A7 and Gelsolin, and provide novel insight into the mechanisms of Clic1 for hepatocarcinoma treatment.     

从质保案例看医院工程质量控制PS十原则

结合医院工程质量保修期维保案例,从人员、材料、工艺、管理等方面总结了医院建筑工程的"质控PS十原则",以期为丰富工程质量管理控制体系提供借鉴。     

Effects of High-Voltage Pulsed Radiofrequency on the Ultrastructure and Nav1.7 Level of the Dorsal Root Ganglion in Rats With Spared Nerve Injury

ObjectivesTo investigate the analgesic effect of high-voltage pulsed radiofrequency (HV-PRF) on the dorsal root ganglion (DRG) for neuropathic pain induced by spared nerve injury (SNI) in rats, especially the influence of this treatment on the DRG ultrastructure and voltage-gated sodium channel 1.7 (Nav1.7) level in the DRG.Materials and MethodsOne hundred fifty adult male Sprague-Dawley rats were randomly divided into five groups: Sham, SNI, Free-PRF, standard-voltage PRF (SV-PRF), and HV-PRF. The 45V-PRF and 85V-PRF procedures applied to the left L5 DRG were performed in SV-PRF group and the HV-PRF group, respectively, on day 7 after SNI, whereas no PRF was concurrently delivered in Free-PRF group. The paw mechanical withdrawal threshold (PMWT) was detected before SNI (baseline) and on days 1, 3, 7, 8, 10, 14, and 21. The changes of left L5 DRG ultrastructure were analyzed with transmission electron microscopy on days 14 and 21. The expression levels of Nav1.7 in left L5 DRG were detected by immunofluorescence and Western blot.ResultsCompared with the Free-PRF group, PMWT in the SV-PRF group and HV-PRF group were both significantly increased after PRF (all p < 0.05). Meanwhile, the PMWT was significantly higher in the HV-PRF group than that in the SV-PRF group on days 14 and 21 (all p < 0.05). There were statistically significant differences between the SV-PRF and Free-PRF groups (p < 0.05). Similarly, statistically significant difference was found between the HV-PRF and Free-PRF groups (p < 0.05). Especially, comparison of the SV-PRF group and the HV-PRF group revealed statistically significant difference (p < 0.05). The Nav1.7 levels were significantly downregulated in the SV-PRF group and HV-PRF groups compared to that in the Free-PRF group (all p < 0.01). A significantly lower Nav1.7 level was also found in the HV-PRF group compared to that in the SV-PRF group (p < 0.05).ConclusionsThe HV-PRF produces a better analgesic effect than SV-PRF applied to the DRG in SNI rats. The underlying mechanisms may be associated with improving the histopathological prognosis and the downregulation of Nav1.7 levels in the DRG.     

Effect of green coffee extract supplementation on serum adiponectin concentration and lipid profile in patients with non-alcoholic fatty liver disease: A randomized,controlled trial

ObjectivesThe current study evaluated the effects of green coffee extract (GCE) on serum lipid profile and adiponectin levels in patients with nonalcoholic fatty liver disease (NAFLD).DesignThis randomized, double-blind, placebo-controlled clinical trial was conducted on NAFLD patients aged 20–60 years and body mass index (BMI) of 25−35 kg/m².SettingPatients were recruited from the Bahman poly-clinic (Neyshabur, Iran) between January and June 2016.InterventionsThe study subjects were randomly assigned to receive a daily dose of 400 mg GCE (n = 24) or placebo (n = 24) for eight weeks.Main outcome measuresSerum liver enzyme levels, lipid profile, adiponectin concentrations, and hepatic steatosis grade were measured for all patients at baseline and the end of the trial.ResultsGCE supplementation significantly reduced BMI [mean difference (MD): −0.57 and 95 % confidence interval (CI): −0.84 to −0.29, P < 0.001] and increased serum high-density lipoprotein cholesterol (MD: 7.06, 95 % CI: 0.25–13.87, P < 0.05) compared to the control group. Serum total cholesterol decreased significantly within the GCE group (MD: −13.33, 95 % CI: −26.04 to −0.61, P < 0.05). Triglyceride levels reduced significantly in GCE group compared to the placebo group (MD: -37.91; 95 % CI: −72.03 to −3.80; P = 0.03). However, this reduction was not significant when was further adjusted for mean changes in BMI and daily energy intake (MD: -23.43; 95 % CI: −70.92 to 24.06; P = 0.32). Hepatic steatosis grade, liver enzymes, and adiponectin levels did not show significant differences between the two groups after the intervention.ConclusionsGCE supplementation improved serum lipid profile and BMI in individuals with NAFLD. GCE may be useful in controlling NAFLD risk factors.     

以胜任力为核心的“二经四纬”医学类研究生培养模式构建及实践——以滨州医学院为例

“大健康”背景下，对医学人才提出了更高的要求，为提高医学研究生岗位胜任力，滨州医学院紧紧围绕“岗位胜任力”的核心，从研究生导师队伍建设和研究生培养两个“经度”入手，在创新能力、实践能力、学科交叉思维和国际视野4个“纬度”，实施培养模式改革。在研究生导师队伍建设经度，建立健全研究生导师分类遴选考核体系，构建“四位一体” +“思政贯通”的导师培训体系，开展“医学+X”交叉融合工程，组建国际化导师队伍，全面提高研究生导师岗位胜任力。在研究生培养经度，构建科学学位科研素养全周期覆盖、专业学位 “产学研”一体化的研究生分类培养体系，建立“医学+X”医学研究生交叉培养体系和“S-P-R-I国际化培养模式”，提高研究生学科交叉能力，拓宽国际视野，提升研究生岗位胜任力。自新模式实施以来，切实提升了研究生的创新能力、实践能力、学科交叉思维和国际视野，岗位胜任力显著提高，深受就业单位欢迎，就业率近100%，均从事医学科研或临床工作。     

以格林模式为指导的健康宣教对尖锐湿疣患者情绪及自我效能的影响

目的探讨以格林模式为指导的健康宣教对尖锐湿疣患者情绪及自我效能的干预作用。方法将220例尖锐湿疣患者按就诊时间分为两组,各110例。对照组予以常规护理干预,观察组在此基础上实施以格林模式为指导的健康宣教,观察6个月,治疗后随访6个月统计复发率。干预前后采用医院自制尖锐湿疣患者健康知识调查问卷调查健康知识掌握情况,采用焦虑自评量表、抑郁自评量表评定焦虑、抑郁情绪,采用医学应对问卷评定应对方式,采用一般自我效能感量表评定自我效能。干预后采用纽卡斯尔护理满意度量表评定护理满意度。结果干预后观察组尖锐湿疣患者健康知识调查问卷总分及尖锐湿疣疾病知识、自我保健知识评分显著高于对照组(P<0.01),焦虑自评量表、抑郁自评量表评分显著低于对照组(P<0.01)。干预后观察组一般自我效能感量表评分及医学应对问卷的面对、自我效能评分显著高于对照组(P<0.01),回避、屈服评分显著低于对照组(P<0.01)。观察组复发率显著低于对照组(P<0.05),总满意率显著高于对照组(P<0.01)。结论以格林模式为指导的健康宣教能提高尖锐湿疣患者健康知识掌握度及自我效能水平,有效缓解其不良情绪,改变其应对方式,降低复发率,提高患者满意度。     

基于网络药理学中药复方“三药三方”治疗新冠肺炎的分期治疗分析＊

目的 本研究探究中药复方“三药三方”在新冠肺炎治疗发挥药理作用的多成分、多靶点和多通路的分子机理以及复方药味归经的中医联系，探究“三药三方”药理作用的异同。方法 通过ETCM数据库获取“三药三方”的成分、靶点、药味归经，用STRING数据库进行蛋白互作网络构建，用DAVID和Metascape数据库进行靶标功能富集分析，利用Cytoscape软件构建和分析可视化网络图。结果 “三药三方”多重点作用于脾经和肺经，初期和中期复方重点作用于肺经和胃经，重症期复方重点作用于肝经和心经，通用方的温性药比例最大，初期和中期复方寒性药比例最大，“三药三方”药味均以苦味、辛味、甘味为主。共同化合物中Alexandrin（胡萝卜甙）与Nsp（N7-MTase）（非结构蛋白C端鸟嘌呤-N7甲基转移酶），Oleanolic Acid（齐墩果酸）与E protein（ion channel）（包膜小膜蛋白）有良好结合力。复方调控共同之处：包含共同子模块γ-氨基丁酸受体亚基相互作用以及细胞色素、ATP依赖转位酶和核受体的相互作用，参与调控16条生物通路涉及①神经保护作用；②免疫调节以及抗炎症风暴调节；③内分泌调节以及性激素调控；④能量代谢调控；⑤药物代谢；⑥其他通路。不同之处：“三药三方”也存在各自特异调控的通路，金花清感颗粒和莲花清瘟胶囊参与ACE2相关通路Renin-angiotensin system（肾素-血管紧张素系统）的调节，同时6种复方有各自的子模块相互作用蛋白。结论 “三药三方”在治疗新冠病毒体现辨证论治、治病求因、正气存内、已病防变的中医治疗原则，并且通过网络药理学初步揭示“三药三方”功能调控以及异同之处，阐述“三药三方”通过多成分-多靶点-多通路的综合调控之间的异同关系。     

脉血康胶囊联合瑞舒伐他汀对冠脉临界病变患者冠脉病变程度的影响

目的研究脉血康胶囊联合瑞舒伐他汀对冠脉临界病变患者钙库操纵性钙通道蛋白(SOCC)基质交联分子1(STIM1)、钙释放激活钙通道蛋白Orai1及瞬时受体电位通道1(TRPC1)蛋白水平和冠脉病变严重程度的影响。方法将2015年1月—2016年12月北京安贞医院经定量冠脉造影检查确诊冠脉临界病变(BCL)住院患者160例设为临界病变组(BCL组),150例无冠脉病变的受试者为对照组(简称CTR组)。BCL组随机分为常规治疗组(简称RTT组,80例)和联合治疗组(简称CBT组,80例)。RTT组给予生活方式改善、阿司匹林、AECI、β受体阻滞剂及瑞舒伐他汀10 mg等药物。CBT组在常规药物治疗基础上,每日加用瑞舒伐他汀15 mg和脉血康胶囊3.0 g,连续治疗12个月。检测血小板STIM1、Orai1、TRPC1、高敏C-反应蛋白(hs-CRP)水平及TC、LDL-C、HDL-C、TG浓度。评价BCL组平均狭窄程度(MPS)。结果BCL组130例受试者复查冠脉造影。与本组治疗前比较,治疗后CBT组和RTT组血小板STIM1、Orai1、TRPC1水平均降低(P<0.05);CBT组冠脉临界病变的MPS明显减低(59.74±9.72vs.38.92±13.84,P<0.05),RTT组MPS有所下降(58.96±8.67vs.55.43±10.03),但差异无统计学意义(P>0.05)。与RTT组比较,治疗后CBT组血小板STIM1、Orai1、TRPC1、TC、LDL-C、TG、和hs-CRP明显降低(P<0.05,P<0.01),且CBT组MPS差值大于RTT组(P<0.01)。结论脉血康胶囊3.0 g和瑞舒伐他汀15 mg每日联合应用可降低SOCC水平,其可能是冠脉临界病变治疗的优化选择。     

上海市儿科人才队伍建设的困境与对策

目的 缓解儿科人才队伍不足,提出解决现有问题的方法.方法 以系统理论思想为指导,运用文献研究、实证的研究方法.结果 目前政策环境中存在儿童专科医院补偿水平不足,儿科人才来源缺乏,儿科医疗收费水平不能体现儿科医务人员工作价值和儿童医保保障水平低等问题.结论 解决儿科人才建设问题需多层次多角度予以政策扶持,首先制定专门人才规划,其次解决儿科系招生问题,再次解决儿科医务人员待遇问题.